Journal: International Journal of Molecular Sciences
Article Title: Cardiomyogenic Differentiation Potential of Human Dilated Myocardium-Derived Mesenchymal Stem/Stromal Cells: The Impact of HDAC Inhibitor SAHA and Biomimetic Matrices
doi: 10.3390/ijms222312702
Figure Lengend Snippet: Attachment and survival of healthy and pathological/dilated myocardium-derived hmMSC on collagen I-based hydrogelss. ( A ) Healthy and pathological hmMSCs were allowed to attach to the collagen type I (Col) hydrogels, and collagen type I hydrogels with hyaluronic acid (Col-HA) and 2-methacryloyloxyethyl phosphorylcholine (Col-MPC) for 24 h. ( B ) Assessment of cell viability by CCK-8 absorption after 24 h in culture. * Significantly better attachment of hmMSC on Col hydrogels compared to the Col-MPC and Col-HA. Data are shown as absorption mean ± SD and are significant at p ≤ 0.05 from not less than tree repeats ( n = 3) of two patients from each group. Scale bars are 100 µm. Arrows show linear growth mode of healthy hmMSC on Col hydrogel.
Article Snippet: Briefly, 12% ( w / w ) of collagen solution was buffered with MES in a syringe mixing system, then MPC solution (Merck KGaA, Darmstadt, Germany) in MES was added, Col:MPC ( w / w ) ratio was 2:1.
Techniques: Derivative Assay, CCK-8 Assay